Blood vessels serve as crucial transport channels for delivering oxygen and nutrients to cells. The intricate process of vascular network formation involves two fundamental mechanisms: vasculogenesis and angiogenesis. Vasculogenesis entails the de novo production of endothelial cells,while angiogenesis involves the formation of new blood vessels from pre-existing ones. The regulation of angiogenesis is a complex interplay of various signaling pathways.In recent years, there has been growing recognition of the significant role played by biomechanical signals in angiogenesis regulation.Mechanical forces,including shear stress on vascular endothelial cells,static pressure exerted by blood on vessel walls,stretching force caused by blood flow,and extracellular matrix stiffness,exert profound effects on blood vessels.The vascular system detects and responds to these mechanical signals by engaging in a series of signaling pathways to maintain its structural and functional homeostasis.This article provides an overview of how mechanical signals influence vascular development,tumor angiogenesis, and lymphangiogenesis.By comprehensively elucidating the critical role of biomechanical signals as regulators of vascular system development from these three perspectives,we aim to offer valuable insights for the diagnosis and treatment of associated diseases.