多系分化应激耐受(multilineage differentiating stress enduring, Muse)细胞是近年来发现的一种新型干细胞,因其具有相比间充质干细胞更优秀的三胚层分化能力、应激耐受能力、归巢能力和原位分化能力,以及非致瘤性等特点,受到越来越多的研究和关注。Muse细胞在多种疾病的动物模型实验和临床试验中显示出较好的效果,预示着其在再生医学领域的巨大应用潜力。然而, 由于Muse细胞在体内的基础含量极低,传统的培养方法难以在短时间内获得大量高纯度的Muse细胞,这限制了其转化应用。本文旨在探讨Muse细胞的特征和培养方法,以及在疾病模型实验和部分临床试验中的研究进展,以期为Muse细胞的深入研究提供理论依据。

Multilineage differentiating stress enduring (Muse) cells are a newly discovered type of stem cells that have attracted increasing attention due to their superior abilities compared to mesenchymal stem cells, such as enhanced tri-germ layer differentiation potential, stress tolerance, homing ability, in situ differentiation capacity, and non-tumorigenicity. Muse cells have shown promising effects in various animal disease models and clinical trials, highlighting their immense potential for applications in the field of regenerative medicine. However, the extremely low baseline levels of Muse cells in vivo pose a challenge for conventional culture methods, which often fail to obtain large quantities of high-purity Muse cells within a short time frame, limiting their translational applications. This article aims to discuss the characteristics and culture methods of Muse cells, as well as research progress in disease models and specific clinical trials, providing a theoretical basis for further studies on Muse cells.